FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP9..ph-vec_Col..h_B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP9..ph-vec_Col..h_B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109661
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT13361.2183000337403451TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC4480.4085317478410739TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA2020.18420404701762705TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1720.15684701033184084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCTA150.0022198769.9972723
GCCGTCT2250.040.44286351
TGCCGTC2450.038.56992350
ATGCCGT2500.037.7985249
CTCGTAT2500.036.39857544
CTGCTTG2450.035.7128959
TCGTATG2650.035.6589845
CAGTCAC2650.035.6589827
CGTATGC2700.034.9986346
CCGTCTT2600.034.9986352
CTTCTGC2500.034.9986356
GATCTCG2600.034.9986341
ACATTAC2950.034.40543432
CATTACT2950.034.40543433
CGTCTTC2750.034.36229353
TATGCCG2750.034.36229348
GTATGCC2750.034.36229347
CGATCTC2650.034.3382840
ATCTCGT2750.033.0896242
TCTTCTG2750.033.0896255
Produced by FastQC (version 0.11.5)