FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP9..ph-bZIP63_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP9..ph-bZIP63_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380142
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC10070.2649010106749583TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT8180.21518274749961855TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3000.036.16564645
GCCGTCT3050.034.4252648
ATGCCGT3200.033.90529646
CGTATGC3250.033.38367543
TGCCGTC3250.033.38367547
GTATGCC3300.032.8778644
CACAGTA450.00430269331.11432629
GATCTCG3300.030.75670838
CTCGTAT3450.030.43392441
TCGTATG3700.029.32349842
TTACTCG3850.029.09008832
ATCTCGT3500.028.99918439
CGATCTC3450.028.40499537
TCTCGTA3500.027.99921240
CCGTCTT3850.027.27195749
CACATTA4200.025.83600428
TACTCGA4200.025.83260533
CTGCTTG3850.025.45382756
ATTACTC4550.025.383931
CAGTCAC4550.023.8486224
Produced by FastQC (version 0.11.5)