FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP9..ph-bZIP25_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP9..ph-bZIP25_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707408
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC15430.21812023612964512TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT10630.1502668898287834TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.033.20472348
GCCGTCT3700.032.1617751
CGTATGC4150.032.04780646
ATGCCGT4050.031.9749249
GTATGCC4350.029.76975347
TGCCGTC4200.029.16631550
CCGTCTT4750.027.26282952
CTCGTAT4750.026.52599544
TCGTATG5300.025.09403645
CTGCTTG4800.023.33305259
CGATCTC5100.023.33305240
GATCTCG5250.022.66639141
TCTCGTA5750.021.91277743
TTACTCG6200.021.45135535
CTCGATC5800.020.51699338
AAGGGGG2101.9608706E-919.9997670
ATCTCGT5950.019.99975842
TACTCGA6900.019.27512736
CGTCTTC6450.018.99201853
TAGCGAG950.007208435318.4208324
Produced by FastQC (version 0.11.5)