FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP9..ph-bZIP10_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP9..ph-bZIP10_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826980
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC13190.15949599748482432TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT10010.12104283054003724TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.025.99973145
GCCGTCT3250.025.84588648
TGCCGTC3650.024.9312547
GTATGCC3800.024.86816444
TCGTATG4100.023.04854242
ATGCCGT3950.023.03773746
CGTATGC4200.022.4997743
CTCGTAT3850.021.81795544
CCGTCTT4300.019.53468149
CGATCTC4500.018.66647340
GATCTCG4600.017.49981938
TCTCGTA5000.016.09983340
TTACTCG5900.016.01678332
GACACCT1554.053749E-415.80628866
CAGTCAC6150.015.36569527
ATCTCGT5450.014.7704939
ACTCGAT7150.014.19565734
CGTCTTC5500.013.99985550
CTGCTTG4851.8189894E-1213.71119856
CCAGTCA7500.013.0665323
Produced by FastQC (version 0.11.5)