FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP63..ph-vec_Col..h_B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP63..ph-vec_Col..h_B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129661
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT14891.148379235082253TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC4770.3678824010303792TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA2140.1650457732085978TruSeq Adapter, Index 27 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1770.13650982176598978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCTC150.002220442570.0000135
CGTGGGT200.006938203252.528
CCGAAGA200.006938203252.533
GCTACTC200.006938203252.556
TGTACCT200.006938203252.52
GCCGTCT2050.051.21951351
TGCCGTC2100.049.99999650
CGTATGC2250.049.7777846
ATGCCGT2150.048.8372149
TATGCCG2300.047.17391648
GTATGCC2350.046.1702147
TCGTATG2400.045.20833645
CTCGTAT2350.044.6808544
CCGTCTT2400.043.75000452
TGCTTGA2250.043.55555760
CTGCTTG2200.042.95454859
GATCTCG2150.042.3255841
CGTCTTC2500.042.00000453
TCTCGTA2350.041.7021343
ATCTCGT2200.041.3636442
Produced by FastQC (version 0.11.5)