FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP63..ph-bZIP9_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP63..ph-bZIP9_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453397
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC8070.17798970879824966TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT5610.12373262284487987TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTGT252.3897298E-455.9988543
TGCCGTC1800.033.0548847
GTATGCC2100.029.99938644
ATGCCGT2150.029.30172546
TACCCGG604.9801846E-429.1660754
TATGCCG2350.028.29729345
GCCGTCT2000.027.99942648
CGTATGC2200.027.04489943
CCGTCTT2350.025.3186349
TCGTATG2500.025.19948442
GTTCTAC952.7781934E-422.1048146
AGCGGGA952.7781934E-422.1048156
CTCGTAT2750.021.6359241
CGATCTC2700.020.74031637
CAGTCAC3350.019.8525324
GCGGAAC900.00527265819.44404632
GATCTCG3001.8189894E-1218.66628338
TCTCGTA3200.018.59336940
TTACTCG3600.018.47184432
CGTCTTC3101.8189894E-1218.06414650
Produced by FastQC (version 0.11.5)