FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP53..ph-bZIP9_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP53..ph-bZIP9_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856011
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG12470.1456756980926647TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG12050.14076921908713788TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT1850.039.72886741
TCGGGGG5000.037.09919740
ATCGGGG5650.034.68951439
AATCGGG6050.032.39599238
ACTCCGG7450.028.65709529
CGGGGGG4100.028.17011841
CACTCCG7500.027.53273428
TCCGGAG7650.027.45038231
GAATCGG7550.026.42326537
CTCCGGA7950.026.4145230
CGGAGAA8900.024.77474433
CCGGAGA8750.024.39947132
GTCACTC9150.023.33282926
TCACTCC9400.022.71227527
CCAGTCA9350.022.45940823
AGAATCG9050.022.43045236
CAGTCAC9450.021.8513824
TCCAGTC9950.021.10507222
GGAGAAT10400.020.86493334
AGTCACT10100.020.7916325
Produced by FastQC (version 0.11.5)