FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP53..ph-bZIP63_Col..s-B..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP53..ph-bZIP63_Col..s-B..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309187
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3651.7267503E-611.50669149
TGCCGTC3803.7605193E-49.21039950
GCCGTCT3450.00160075559.13030851
TATGCCG4250.00114586438.2351848
AACCCGC4050.00681523757.77790616
CGTATGC4955.96504E-47.777670446
ATGTACG4700.0030846577.446706366
AATCGCC5804.0317327E-47.2420452
CCGTCTT5550.00201266426.936840552
TAGTTCG5200.008238826.73067631
AGTCACG9052.7705473E-45.801024466
GTCACGA8900.00140377195.505541368
TCACGAG11001.18062555E-45.40901669
ACGTCTG24503.6379788E-125.28564170
GAACTCC22808.731149E-115.219226468
Produced by FastQC (version 0.11.5)