FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP53..ph-bZIP63_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP53..ph-bZIP63_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928826
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.030.67362445
CGTATGC5250.025.99954843
ATGCCGT5400.024.62920246
GTATGCC5850.024.52948844
CCGCTCA6700.022.46229730
CGCTCAT6400.021.8746231
TCGTATG7150.020.55908242
GCCGTCT6350.020.39334748
TGCCGTC6550.020.3049947
CTCGTAT7850.018.7257941
ACCGCTC7850.017.83408529
TATCTCG8350.017.60448538
TCTCGTA8850.017.00535440
CTGCTTG7950.016.72926956
CACCGCT8600.016.68575728
CCGTCTT8850.016.21440749
GCTCATT10050.014.97486432
TCACCGC9850.014.568274527
ACTCCAG10200.014.06874720
ATCTCGT11350.013.8764139
Produced by FastQC (version 0.11.5)