FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP53..ph-bZIP25_Col..h-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP53..ph-bZIP25_Col..h-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719465
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG7770.10799691437387504TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG7240.10063032948093376TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG4000.031.49962443
ATCGGGG4250.031.29374542
GCGCAAT450.00430765231.1107432
GCGGTCA500.007209023527.99966617
AATCGGG5000.027.29967341
CGGGGGT1800.027.22189944
GAATCGG5550.025.85554740
AGGGGGG959.382213E-625.7927511
CACTCCG5700.024.56111131
ACTCCGG5800.024.13764432
CGGGGGG3450.023.33305544
CTCCGGA6450.022.24779533
TCCGGAG6500.022.0766634
GTCACTC6550.021.90813629
AGAATCG6550.021.90813639
CCGTTGC800.002666371421.87473942
TCACTCC6650.021.05238230
CCGGAGA7000.020.9997535
CAGTCAC7400.019.3916627
TCCAGTC7700.018.181625
Produced by FastQC (version 0.11.5)