FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP53..ph-bZIP10_Col..s-B..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP53..ph-bZIP10_Col..s-B..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015360
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT1452.440666E-416.89637251
CGTGCGG1300.00229176616.15367351
TAGCGGT1708.1795314E-414.41161234
AGCCCCG2000.002782886812.2498739
GCGAGCT2000.002782886812.2498732
GGAGGAC5050.0062048286.930619260
CGTCTGA11853.7773134E-86.49782270
ACGTCTG11802.387751E-76.22874869
ACTCCAG10455.4288812E-55.69371970
CCAACTG7400.00628259825.67631962
GACTATT7650.0090751995.49013816
CTAAGAG8300.0036936495.481869768
CACGTCT13854.646612E-65.306802768
ACACGTC14106.4365486E-65.21271167
GCACACG15451.1561278E-65.21030169
CACACGT15305.279164E-65.032626670
Produced by FastQC (version 0.11.5)