FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP53..ph-bZIP10_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP53..ph-bZIP10_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641736
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG7670.11951955321191268TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG7170.11172818729197054TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG3200.034.99949639
TCGGGGG3300.032.87831540
CGGGGGG2550.027.45058341
AATCGGG4300.026.86007738
ACTCCGG4950.025.45417829
CACTCCG5150.024.46566628
GAATCGG4750.024.31543737
CGGGGGT1451.7265847E-824.1375841
TCCGGAG5250.023.99965331
CAGTCAC5450.023.12073324
CCGGAGA5500.022.90876232
CTCCGGA5600.022.49967430
GTCACTC5800.021.72551526
CCAGTCA5800.021.72551523
CGGAGAA6200.020.32228733
AGTCACT6450.020.07879425
CTCCAGT6550.019.77224721
TCCAGTC6400.019.6887522
CTCGTGA900.00527481819.44416217
TCACTCC6550.019.2363627
Produced by FastQC (version 0.11.5)