FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP25..ph-vec_Col..h_B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP25..ph-vec_Col..h_B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146654
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG8150.5557298130293071TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT4400.30002591132870565TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG1690.11523722503307104TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG1590.10841845432105501TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCA150.002221025570.043
GGACGGC200.0069400152.561
CGGGGGT1000.049.00000444
ATCGGGG2300.048.69565642
TCGGGGG2200.047.72727243
GGGTGGC305.862548E-446.66666447
AATCGGG2400.046.66666441
CACTCCG2950.046.27118731
TCCGGAG3100.044.03225734
TCACTCC3100.044.03225730
ACTCCGG3150.043.33333232
AGAATCG2750.043.27272839
CGGAGAA3150.042.2222236
GTCACTC3250.042.029
GGAGAAT3200.041.562537
CTCCGGA3350.040.7462733
TCCAGTC3350.040.7462725
CAGTCAC3350.040.7462727
AGTCACT3400.040.14705728
CCAGTCA3400.040.14705726
Produced by FastQC (version 0.11.5)