FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP25..ph-bZIP9_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP25..ph-bZIP9_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543715
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC17080.3141351627231178TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT12420.22842849654690417TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5000.034.299145
GCCGTCT4800.034.2699348
ATGCCGT5050.033.95950346
TGCCGTC5050.033.95950347
CGTATGC5100.033.62656443
GTATGCC5100.033.62656444
AAGGGGG1109.058567E-1031.81734870
CTCGTAT5500.029.90830441
CCGTCTT5500.029.90830449
CGATCTC5400.029.165937
TCGTATG6200.027.66056442
CGTCTTC5700.027.63085450
GATCTCG5600.027.49927938
CTGCTTG5250.026.66596856
TTACTCG6600.025.98416532
CTTCTGC5400.025.92524553
ATCTCGT6250.024.63935339
TACTCGA7100.023.6613533
TCTCGTA6800.023.16115640
ACTCGAT7100.022.6754634
Produced by FastQC (version 0.11.5)