FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP10..ph-vec_Col..h_B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP10..ph-vec_Col..h_B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154541
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG9660.6250768404501071TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT4420.2860082437670262TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG2060.13329795976472264TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG2000.12941549491720644TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT352.0790154E-550.044
ATCGGGG1850.043.51351542
TCGGGGG1650.040.30303243
CACTCCG2400.037.91666431
ACTCCGG2450.037.14285732
TCACTCC2500.036.430
AATCGGG2250.035.7777841
AGAATCG2350.035.74468239
GTCACTC2450.035.71428729
TCCGGAG2600.035.00000434
GTCCGAA400.002412979335.014
CTCCAGT2550.034.31372524
TCCAGTC2550.034.31372525
CTCCGGA2700.033.70370533
GAATCGG2400.033.54166440
GGAGAAT2850.031.92982537
CCGGAGA2900.031.3793135
CCAGTCA2800.031.2526
TGCTTGC450.00429703931.11110960
CGGAGAA2950.030.84745636
Produced by FastQC (version 0.11.5)