FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_ps-bZIP10..ph-bZIP9_Col..h-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_ps-bZIP10..ph-bZIP9_Col..h-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475801
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC7780.16351373788621712TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT6100.12820485875397486TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1750.033.99964545
GTATGCC1750.033.99964544
CGTATGC1800.033.0552143
GCCGTCT1700.032.9408348
TGCCGTC1850.032.16182347
ATGCCGT2150.027.67412646
GATCTCG2200.025.45427738
CTCGTAT2250.024.88862841
CCGTCTT2300.024.3475749
TCGTATG2400.023.33308842
TCTCGTA2450.022.85690140
CGTCTTC2300.022.82584850
CGATCTC2650.022.45259337
CTGCTTG2350.022.34018956
ATCTCGT3150.018.88868939
TTACTCG3500.017.99981132
GTCACAT3651.8189894E-1216.30119926
CCAGTCA3651.8189894E-1216.30119923
CTCGATC3451.4551915E-1116.23171435
GCTTGAA3701.8189894E-1216.08091258
Produced by FastQC (version 0.11.5)