FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_bZIP63_Col-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_bZIP63_Col-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125028
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC1790.14316793038359407TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1390.11117509677832166TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAG207.901048E-570.068
CCAATCG200.00693762352.520
TCTACCC200.00693762352.550
CACAGAT200.00693762352.544
CCCAATC400.002411221635.019
ATGCCGT450.00429392831.11111346
TGCCGTC500.007186194427.99999847
AAAGGGG657.919504E-426.92307769
CAATCGA657.919504E-426.92307721
CCAGTCA959.3106955E-625.78947323
CATTCAG1101.0048152E-625.45454430
ACTCCAG1253.055633E-622.420
CAGTCAC1102.8966497E-522.27272624
AGTCACA1154.0818733E-521.30434825
TCCAGTC1154.0818733E-521.30434822
GTCACAT1154.0818733E-521.30434826
CTCCAGT1355.9542162E-620.7407421
AACTCCA1355.9542162E-620.7407419
GAACTCC1205.6656478E-520.41666818
CACATTC1301.04808234E-418.84615328
Produced by FastQC (version 0.11.5)