FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_bZIP53_Col-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_bZIP53_Col-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468005
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC4904.5731213E-810.71408144
TATGCCG3904.4913766E-59.87160745
AGTTCGC3050.00598888249.18015266
ATGCCGT4502.1376186E-48.55539246
CATTCAG10709.094947E-128.17741430
TCGTATG6101.0210337E-58.03263342
GAACTCC11051.546141E-107.60166418
CGTATGC6108.860127E-57.458873743
CTGAACT10051.12449925E-87.31329316
TGCCGTC5854.4531262E-47.1793547
ACTCCAG10955.804577E-86.712608320
TCTGAAC12753.4760888E-96.58810915
CTCCAGT11753.1934178E-86.553464421
CAGTCAC12802.6029738E-86.288942327
TCCAGTC11752.2039967E-76.2555822
CACATTC16003.110472E-106.124882728
ACGTCTG14002.561137E-85.999885612
GAGCACA13353.889454E-75.7684986
ACATTCA17001.371518E-95.76459629
CTCGTAT7300.0054132665.753314541
Produced by FastQC (version 0.11.5)