FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA7_PN-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA7_PN-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32361757
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36000.015.16638648
TGCCGTC29200.014.74287750
GCCGTCT26600.013.42080351
CGTATGC42250.013.33702946
ATGCCGT38050.012.87755349
CGTATCT69050.010.49223539
TCGTATC90800.09.86770438
TATCTCG73400.09.53660741
CTCGTAT68150.08.98736144
TATCGCG16050.08.94064341
TCGTATG66200.08.564795545
CCGTCTT49400.08.43101852
TCTCGTA72850.08.40753243
ATTCGTA118150.08.23519236
TTCGTAT124250.07.830888337
CGCGTAT13200.07.689251444
GTATGCC77100.07.671712447
ATCTCGT91350.07.39449942
CGTATCG26250.07.19988839
GAATTCG142350.07.0319234
Produced by FastQC (version 0.11.5)