FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA7_PN-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA7_PN-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31084098
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31750.014.77142748
TGCCGTC28700.012.92663150
GCCGTCT25300.012.0353951
CGTATGC41650.011.176346
ATGCCGT38700.010.94298649
CGTATCT65650.09.2763439
TCGTATC88300.08.95797538
TATCTCG67750.08.26555641
TATCGCG14100.07.94314141
ATTCGTA109950.07.51239136
CCGTCTT46850.07.395819752
CTCGTAT67900.07.371010344
TCGTATG67900.07.21638545
TTCGTAT117850.07.187003637
ATCTCGT81850.07.012721542
TCTCGTA71950.06.712892543
ATCGCGT13802.8558134E-96.34048342
CGTATCG25700.06.264506339
GTATGCC78200.06.17637747
CGCGTAT11509.955111E-76.086854544
Produced by FastQC (version 0.11.5)