FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA7_CS-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA7_CS-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22511905
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36850.017.76094248
TGCCGTC32900.017.5529450
ATGCCGT36950.016.5762249
GCCGTCT27750.016.5222951
CGTATGC41800.015.49021146
CCGTCTT43150.012.08552352
CTCGTAT58400.011.50663544
TCGTATG58200.011.42595545
AATCTCG74950.010.32008941
TCTCGTA64500.010.30993143
GTATGCC65400.010.22156747
CAGTCAC125350.09.77247627
AATCGCG14000.09.74988340
ATCGCGT9850.09.59379441
ATCTCGT75250.09.5812642
CCAGTCA134150.09.2098126
TCCAGTC140800.08.79972825
CATTCAG146050.08.79478833
ACTCCAG151150.08.31294523
CTCCAGT147800.08.31191924
Produced by FastQC (version 0.11.5)