FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA1_PN-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA1_PN-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32287786
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32550.016.4513250
TATGCCG41850.015.7225448
GCCGTCT32250.014.75942851
ATGCCGT42300.013.98320349
CGTATGC46950.013.79102246
CGTATCT74900.012.24277439
TCGTATC99150.011.08403638
TATCTCG79400.010.57915841
CCGTCTT53450.09.8875752
CTCGTAT72700.09.48401444
TCGTATG71650.09.13452145
ATTCGTA130400.09.0987836
TTCGTAT135650.08.79823637
TCTCGTA80850.08.78772343
ATCTCGT91600.08.5970142
GTATGCC86200.08.07990447
CGCGTAT13700.07.919567644
TCGCGTA13300.07.894596643
TATCGCG18250.07.862874541
GTATCTC120750.07.391173440
Produced by FastQC (version 0.11.5)