FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA1_PN-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA1_PN-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24534383
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28950.014.024013548
TGCCGTC21650.013.41785550
CGTATGC32600.011.91703746
GCCGTCT20350.011.86718251
ATGCCGT30050.011.64711849
CGTATCT51200.09.63857739
TCGTATC66150.09.47080738
TATCGCG11050.09.18543141
TATCTCG54550.08.66169441
CTCGTAT52800.08.15327944
AGGGGGG75800.08.1272151
TCGTATG50650.07.94659945
ATTCGTA87850.07.728976736
TCTCGTA58550.07.35260343
TTCGTAT91100.07.338018437
CCGTCTT37050.07.179373352
CGTATCG19150.07.12786839
ATCGCGT11452.6011548E-97.030484742
GTATGCC62800.06.91074647
ATCTCGT66600.06.62154442
Produced by FastQC (version 0.11.5)