FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA1_CS-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA1_CS-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29595846
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60600.022.46666348
TGCCGTC48750.022.32787150
GCCGTCT43700.021.3841251
CGTATGC66000.020.94665746
ATGCCGT60250.019.63456249
CGTATCT101300.019.2445339
TCGTATC127350.018.33112738
TATCTCG102150.017.40551641
CTCGTAT91950.016.44344744
TCGTATG88100.016.28806745
ATTCGTA148850.016.10657936
TTCGTAT153900.015.76002637
TCTCGTA98050.015.49186543
CCGTCTT67050.014.98110952
GTATGCC97450.014.90485747
GTCACGA166550.014.52104729
AGTCACG169000.014.35193128
GAATTCG171500.014.22430134
ATCTCGT121100.014.10383542
CGAATTC176550.013.95620233
Produced by FastQC (version 0.11.5)