FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYBA1_CS-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYBA1_CS-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26258796
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41150.024.41025248
TGCCGTC34800.022.72947350
CGTATGC46300.021.92184846
ATGCCGT41750.020.87387549
GCCGTCT31350.020.54189351
CGTATCT68800.017.75410739
TCGTATC87600.017.49981738
TATCGCG15150.017.0954341
TATCTCG73600.016.3584341
ATCGCGT13200.015.64365742
GTCACGA105050.015.35919229
AGTCACG105100.015.28531128
CTCGTAT68350.015.15700444
ATTCGTA111000.014.72507136
TCGTATG67550.014.55932245
CCGTCTT48800.014.200562552
TCTCGTA72250.013.951303543
TTCGTAT116450.013.88564237
GTATGCC77700.013.7384947
TCACGAA119100.013.66481930
Produced by FastQC (version 0.11.5)