FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYB15_PN-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYB15_PN-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30189185
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC35950.020.54211450
GCCGTCT34950.019.72788851
TATGCCG40750.019.58253148
ATGCCGT45100.016.76249949
CGTATGC50300.015.65583146
CCGTCTT56700.012.90152
TCGTATG70500.011.41826945
CTCGTAT71800.011.26025944
TCTCGTA75050.010.63280543
TATCTCG73600.010.31919141
GTATGCC79350.010.14477647
CGTCTTC72000.09.77065553
ACCTACC184700.09.22919751
ATCTCGT87100.08.960875542
ACCTACT166700.08.2939861
ACCAACC293050.08.1937791
ACCTAAC189900.07.48346851
GCTATCT106500.07.361465539
AACTACC134650.07.1227231
ACCAACT245250.06.3368851
Produced by FastQC (version 0.11.5)