FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYB15_PN-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYB15_PN-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28057293
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37100.020.3770148
GCCGTCT33000.019.83299451
TGCCGTC36700.019.45470650
CGTATGC44550.017.28365346
ATGCCGT42900.017.13257249
CCGTCTT53600.012.79824352
TCGTATG63000.012.38867745
CTCGTAT65300.011.79148644
TCTCGTA66750.011.16836743
TATCTCG68700.010.74947141
GTATGCC73900.010.70346947
ACCTACC148600.010.4350251
CGTCTTC67000.010.08188153
ATCTCGT77200.09.65658842
ACCAACC251150.08.6131871
GCTATCT93050.08.53828839
ACCTACT139450.07.3545761
AGCTATC113850.07.347286738
ACCTAAC149600.07.15975951
CTGCTTG98350.06.47673459
Produced by FastQC (version 0.11.5)