FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYB14_PN-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYB14_PN-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28006679
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32650.016.1865750
TATGCCG39750.015.76075348
GCCGTCT28900.015.74369151
CGTATGC45550.013.36970846
ATGCCGT42400.013.28988149
CGTATCT74950.011.81441739
TATCGCG14150.011.62529741
TATCTCG71850.010.86277341
TCGTATC99950.010.43510538
CCGTCTT46900.010.14909152
TCGTATG64300.09.63437145
ATCGCGT13550.09.55705842
CTCGTAT68300.09.53132644
ATCTCGT86150.08.69401442
ATTCGTA125700.08.57586836
GTATGCC78600.08.5494847
TTCGTAT127500.08.53716337
TCTCGTA76550.08.45840343
CGCGTAT12400.07.903098644
TCGCGTA11607.2759576E-127.844715643
Produced by FastQC (version 0.11.5)