FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvMYB14_PN-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvMYB14_PN-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33143038
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52400.022.04169548
TGCCGTC43200.021.71267950
GCCGTCT40250.020.52147151
CGTATGC57700.019.65315246
ATGCCGT53950.018.42422549
CGTATCT91400.016.0447239
TATCGCG19950.015.7892941
TCGTATC120350.015.61684138
TATCTCG92850.015.0025241
CTCGTAT83050.014.32846544
ATCGCGT18600.013.5482342
TCTCGTA91600.013.18216143
CCGTCTT64950.013.14832552
TCGTATG84150.013.143043545
GTATGCC94550.012.84490647
ATCTCGT106950.012.43557442
TTCGTAT157650.012.38811437
ATTCGTA159150.012.29327236
TCGCGTA15100.012.28462643
CGCGTAT14450.011.86830244
Produced by FastQC (version 0.11.5)