FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvHY5_CS-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvHY5_CS-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74266
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCC150.00221720269.99596440
CGAGAAA207.882205E-569.99596414
AGGGGAA150.00221720269.99596463
TTCATCG150.00221720269.99596450
TAGCACA252.3692186E-456.03455
GGTCGTT200.006909713652.5323454
CGTGAGT200.006909713652.5323451
GTCGTTT200.006909713652.5323455
CCTTCCG200.00692816352.4969751
TGCCCGG200.00692816352.4969724
CGAGTCC200.00692816352.4969766
ACGTTTC200.00692816352.4969715
CTTCCGA200.00692816352.4969752
CAACGCT200.00692816352.4969738
GGCAAGC200.00692816352.4969730
ATACTAC200.00692816352.4969756
CGCTAAT200.00692816352.4969741
AACGCTA200.00692816352.4969739
GTGTCAA200.00692816352.4969726
ACGCTAA200.00692816352.4969740
Produced by FastQC (version 0.11.5)