FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_VvHY5_CS-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_VvHY5_CS-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64888
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAG150.002215423670.0000130
ACCTGGC150.002215423670.0000118
AAGCCCC150.002215423670.0000157
GTGTCCG150.002215423670.0000150
GCTAGAT150.002215423670.0000112
CAGCTAG150.002215423670.0000110
GTAAGGC150.002215423670.0000148
CCACGTA150.002215423670.0000167
AGAGCGC150.002215423670.0000160
GGCAGGC200.006922642752.522
GCCCAGC200.006922642752.531
CGTATCG200.006922642752.570
TGCCACG200.006922642752.565
AGCCCCT200.006922642752.558
CGGCAAA200.006922642752.529
ATTACCC200.006922642752.570
CCAGCCA200.006922642752.533
GTCTAGC200.006922642752.570
ATCGTAT200.006922642752.565
GTACTTT200.006922642752.57
Produced by FastQC (version 0.11.5)