FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtTGA5_NP-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtTGA5_NP-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667665
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC13000.017.49966862
CGGCAGT15600.015.256233
CCGGCAG16400.014.29858332
ACGGTGG17950.013.25880161
CTCAACG18650.012.94881657
CACCGGC18650.012.57355330
CGGCTTA19400.012.08758722
GCCGTCT7700.011.81795851
AACGGTG23300.010.96545760
CTCCGGC21850.010.89252419
TCCGGCT23050.010.7809320
CCGGCTT22450.010.757256521
TATGCCG9450.010.74053748
GGCAGTC22750.010.61523834
ACCGGCA22500.010.57763131
CAGTCTG25000.010.0798636
TGCCGTC9500.09.9471850
TCACCGG24400.09.89745529
AGTCTGC24050.09.89591337
CCTCCGG25150.09.60240318
Produced by FastQC (version 0.11.5)