FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtTGA5_CS-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtTGA5_CS-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19800632
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC55300.021.01232162
TATGCCG26200.019.6370948
GCCGTCT24100.019.31504251
ACGGTGG67300.017.52572461
ATGCCGT30400.016.92407249
TGCCGTC30200.016.80436750
GGCAACT76650.015.93581866
CGTATGC35750.014.39137846
AACGGTG82750.014.21125260
CTCAACG83050.013.94920557
GCAACTA89650.013.4688367
TGGCAAC94150.012.93659565
CCGTCTT37300.012.7611952
GCCACCG35650.012.56641970
TCGTATG44950.012.38022445
CTCGTAT43950.011.94520144
GGGTCGC17700.011.6664870
TCTCGTA47550.010.96722743
GTGGCAA114450.010.79493464
GGTGGCA113450.010.70498563
Produced by FastQC (version 0.11.5)