FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtOFP16_Col-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtOFP16_Col-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169346
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5840.34485609344182916TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC3490.206086946252052TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA150.002221619770.0000155
CGCGGTT200.00694185352.554
GGGTATC200.00694185352.554
TCCCGGC200.00694185352.523
GCCGTCT708.6418004E-735.051
TGCCGTC1102.3646862E-1135.050
ATGCCGT1108.949428E-1031.8181849
GTATGCC1001.2469172E-831.50000244
TATGCCG1052.005072E-829.99999845
CATTCAG2400.029.16666833
TCGTATG1202.2719178E-929.16666842
AATCGCG500.00719328628.00000241
CTCGTAT1253.5142875E-928.041
CGTATGC1154.852882E-827.39130443
ATCGCGT657.931184E-426.92307742
AATCTCG1705.456968E-1226.76470841
AGTCACA2650.026.41509428
CAGTCAC2650.026.41509427
GTCACAT2700.025.92592629
CACATTC2850.025.78947431
Produced by FastQC (version 0.11.5)