FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtOFP12_Col-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtOFP12_Col-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264850
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5880.22201245988295262TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC5820.21974702661884085TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTC150.002223151769.998868
CGGCGTC200.00694660652.4991553
GCCGTCT708.667448E-734.99943548
ACCCTAT500.00720107927.9995547
TGCCGTC1056.744576E-726.66623347
ATGCCGT1001.3936589E-524.49960546
CGTATGC901.9236364E-423.33295643
CTCGTAT1052.0332107E-523.33295441
TCGTATG1052.0332107E-523.33295442
TATGCCG952.7738884E-422.10490645
CTCCAGT2950.021.35558521
GTATGCC1003.9230444E-420.99966244
CCGTCTT1355.9967115E-620.74040449
ACTCCAG2700.020.74040420
CATTCAG2800.019.99967630
TCCAGTC2850.019.64880622
GTCACAT2900.019.31003226
CCAGTCA2951.8189894E-1218.98274223
TCTCGTA1301.0538651E-418.8458540
GAACTCC3350.018.80566618
Produced by FastQC (version 0.11.5)