FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtMYB16_Col-B_q.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtMYB16_Col-B_q.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208593
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5190.24880988336137838TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC4470.21429290532280565TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC459.176016E-538.88888550
AAGGGGG556.6728826E-638.18181670
AGGACCT400.002414910835.020
AAGCGCC400.002414910835.065
TATGCCG1800.034.99999645
ATGCCGT1700.032.94117746
CTTCGGC552.98512E-431.8181856
CGTATGC2200.030.22727243
TGCCGTC1750.030.047
GTATGCC2100.029.99999844
CTCGTAT2200.028.63636241
GTGGGGG500.00719705428.00000254
CCGTCGT500.00719705428.00000249
CCAGTCA3650.027.80821823
CTGCTTG1403.9108272E-1027.559
CCGGCTT657.937389E-426.92307952
AATCTCG2500.026.59999838
CAGTCAC3850.026.36363624
CATTCAG3750.026.13333530
GCCGTCT1757.2759576E-1226.048
Produced by FastQC (version 0.11.5)