FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtMYB113_Col-B_q2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtMYB113_Col-B_q2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285108
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT6070.2129017775720078TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC5740.2013272163531013TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1051.2732926E-1136.66666451
CCGTCTT1600.030.62552
TGCCGTC1352.564775E-1028.51851850
CGTATGC1455.9117156E-1026.55172346
GTATGCC1455.9117156E-1026.55172347
ATGCCGT1508.749339E-1025.66666849
GTCTTGG700.001224293125.054
CAGTCAC3000.024.50000227
TATGCCG1601.8499122E-924.062548
CCAGTCA3150.023.33333226
CATTCAG3350.021.940333
TCCAGTC3450.021.30434825
GTCACAT3500.021.029
CTCGTAT1859.888026E-920.8108144
GCGTGAA850.003783879620.58823661
AGTCACA3600.020.41666628
CGTCTTC1901.3440513E-820.26315753
CTCCAGT3700.019.86486424
ATTCAGA3950.019.4936734
TCGTATG1801.6916601E-719.44444345
Produced by FastQC (version 0.11.5)