FastQCFastQC Report
Wed 26 Aug 2020
HL2MTBGXG_n01_AtMYB113_Col-B_q1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2MTBGXG_n01_AtMYB113_Col-B_q1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408995
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT6850.16748371006980525TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC6600.1613711659066737TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGG150.002223876870.068
GCGCGGG200.00694885252.569
GCCGTCT1400.040.048
TGCCGTC1550.038.38709647
TATGCCG1900.035.045
TCGTATG2000.033.25000442
CGTATGC1900.033.15789443
ATGCCGT2050.032.43902646
CTCGTAT2050.030.73170941
GTATGCC2200.030.22727444
CCGTCTT2100.028.33333649
CGCGGGT500.00720502228.00000270
AGCCTAC500.00720502228.00000227
CAGTCAC4550.026.15384524
TCTCGTA2550.023.33333240
CATTCAG5100.022.64705730
TCCAGTC5100.022.64705722
CTCCAGT5000.022.421
ACTCCAG5100.021.96078320
GGGTGTA800.002664238721.87561
Produced by FastQC (version 0.11.5)