FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14128474
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG534710.37846267049081167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGG34400.015.9389631
AGGGGGG39600.013.4784492
AAGGCCC26053.6379788E-127.603288145
GCACACG139250.06.5850673145
CACGTCT120550.06.511214145
ACACGTC123700.06.167498145
GATCGGA380600.06.109925145
AGCACAC155550.05.895022145
AGAGCAC178200.05.8043933145
AGATCGG402650.05.6842384145
TCGGAAG352550.05.68051145
CACACGT126000.05.5891542145
TAGATCG138650.05.459896140-144
ATCGGAA397800.05.139955140-144
CGGAAGA386700.05.1219463145
ACGTCTG139900.05.0338345145
Produced by FastQC (version 0.11.5)