FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_S3i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_S3i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28637416
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG449500.07.6129417145
GATCGGA345650.07.4251785145
TAGATCG140750.06.902344145
TCGGAAG315100.06.6034927145
ATCGGAA399700.06.4573755145
ACACGTC158300.06.3661094145
AGAGCAC273800.06.355036145
CTAGATC43050.06.2311583145
CACACGT157000.05.781663140-144
AGCACAC253200.05.66946145
GCACACG226200.05.480797145
CACGTCT190750.05.321128145
GAGCACA250550.05.1217575145
GAAGAGC441750.05.0220966145
Produced by FastQC (version 0.11.5)