FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21800882
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC135150.012.713652145
GAGCACC135950.09.065833145
CGGAAGC180300.08.564892145
AGAGCAC1085700.08.086719145
CACACGT808150.07.7958913145
GATCGGA1550800.07.5688353145
CACGTCT742250.07.5503535145
ACACGTC769750.07.5160766145
TCGGAAG1409600.07.401213145
CGTCTGA738050.07.387033145
GAACTCC539950.07.331234145
GCACACG867550.07.2955446145
AGCACAC955250.07.157027145
GAAGAGC1285000.07.1371593145
AGATCGG1642400.07.0804915145
TAGATCG565050.07.0440664145
ATCGGAA1571300.07.027143145
GGAAGAG1403700.06.797036145
TCTGAAC724550.06.714167145
AGTCACC376300.06.7047567145
Produced by FastQC (version 0.11.5)