FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_S2i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_S2i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28822856
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA521650.06.963095145
ATCGGAA575300.06.414566145
TAGATCG205400.06.2476497145
CACACGT275400.06.160214145
AGATCGG617550.05.776128145
TCGGAAG478050.05.763073145
ACACGTC267550.05.636405145
AGAGCAC407850.05.5106783145
GCACACG340250.05.3483453145
AAGAGCC129250.05.272798145
CACGTCT298100.05.058773145
Produced by FastQC (version 0.11.5)