FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29918389
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC184450.09.865971145
GAGCACC195650.08.411836145
AGAGCAC1305150.07.6770144145
GAACTCC640600.07.673394145
GCACACG1027250.07.6718154145
TAGATCG673100.07.561402145
ACACGTC920350.07.5467033145
GAAGAGC1553650.07.517737145
CACACGT949650.07.382571145
AGCACAC1142900.07.345902145
ACGTCTG900950.07.3390346145
AGATCGG1940950.07.2727118145
TCGGAAG1678350.07.1881237145
CGTCTGA879000.07.184128145
ATCGGAA1889800.07.1588054145
GATCGGA1835900.06.9977665145
CACGTCT870550.06.9290605145
CGGAAGC247100.06.9244294145
CAGTCAC491400.06.845854145
ACTCCAG630950.06.745093145
Produced by FastQC (version 0.11.5)