FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_S1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_S1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32494809
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA567650.08.748818145
AGATCGG659800.08.416973145
CACACGT254250.08.01281145
TAGATCG267650.07.7470846145
ACACGTC242650.07.5891457145
TCGGAAG523850.07.5288982145
AGCACAC375100.07.3833895145
GCACACG312050.07.109462145
AGAGCAC426100.06.7889223145
ATCGGAA667700.06.3303347145
CACGTCT265050.06.2092266145
GAAGAGC604300.06.1186705145
GAGCACA422950.05.6738577145
AAGAGCC160750.05.5023513145
GGAAGAG717700.05.283214145
Produced by FastQC (version 0.11.5)