FastQCFastQC Report
Fri 17 May 2019
HL2HVAFXY_n01_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL2HVAFXY_n01_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37546982
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1363550.08.597598
GAGCACA1373850.08.5278449
GAAGAGC1568050.07.58714536
AAGAGCA1588850.07.51518777
TCGGAAG1618800.07.30449963
CGGAAGA1665100.07.1100984
GGAAGAG1725850.06.960645
GATCGGA1690850.06.6090481
ATCGGAA1871000.06.45559262
AAGAGCC235300.06.316511145
AGGGGGG182650.06.24761970-74
AGATCGG1671300.05.9083743145
AAGGGGG187300.05.883493470-74
TAGATCG662200.05.84652145
GCACACG1064650.05.7611456145
AGCACAC1196750.05.7310343145
ACACGTC997200.05.6782446145
CCGATGT532000.05.475662730-34
ACGTCTG964050.05.3169823145
CACACGT1027350.05.2787185145
Produced by FastQC (version 0.11.5)