FastQCFastQC Report
Fri 6 Oct 2017
HKYNJBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYNJBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318089654
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18118730.5696107928112619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG1125250.018.5416536
TATACCG1249350.017.6474255
GTTATAC1217550.017.3160273
TTATACC1348550.015.7699174
GGACCAT594650.015.7134856
TCAGGGG1182600.015.67459918
TTAGTCC588150.015.4538868
TACCGGT1328300.015.4477767
ACCGGTG1265500.015.440828
TAAACCG719300.015.3700644
TAGTCCA635250.015.2054089
GGTATTC1119500.015.15940124
GGCTTCA1233200.014.88178114
GTCCACA653000.014.81898311
AGTCCAC736950.014.80891110
TTCAGGG1239350.014.6606817
CCGGTGG1290700.014.4798869
AACGGGG698850.014.45724915
CCTAAAC782650.014.2777322
GGGGGTA1311150.014.1054321
Produced by FastQC (version 0.11.2)