FastQCFastQC Report
Fri 6 Oct 2017
HKYNJBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYNJBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318089654
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCT5866350.013.9198591
ACACCTT5871600.013.9111622
CACCTTC5877450.013.8774353
CTCCTAC4839300.013.85854
TCGACCA4831750.013.8560142
CCTTCTT4376200.013.8545511
CCTACAA3164700.013.8428091
TTCGACC4823600.013.838931
CCTACGT4838150.013.8342586
CCTTCGT5887000.013.8326445
CCACTGA5018000.013.8326372
CCTCTCT2958800.013.830524
TCAACCG3417950.013.8282261
TCTCCTA4854300.013.8249793
ACGGAAT5899450.013.822472
ACCACTG4904050.013.8222431
CTTCAGC4470400.013.8221721
AACTACC3902600.013.8210926
TCAGAAC3914300.013.812682
AATCCTT4119350.013.8094522
Produced by FastQC (version 0.11.2)