FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665607
Sequences flagged as poor quality0
Sequence length41
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410032938.44440358621877No Hit
GGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371370.3481939658942993No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAA325370.30506468126942987No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAA281960.26436376288756935No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAA280150.26266671929689517No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAA258770.24262097787777107No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAA257300.24124271595606325No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAA249890.23429515075888319No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAA229620.21529013772962008No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAA208990.19594759116851015No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAA199960.18748112507801948No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAA187670.1759581053380272No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAA187080.17540492538305602No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAA180350.1690949235238088No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAA175860.16488513030716395No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA171540.1608347279250023No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAA169240.15867826369375884No Hit
GGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163320.15312771228116695No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAA163120.15294019365236317No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAA158570.14867414484707717No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAA147160.1379762070738215No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAA134280.12590000737885804No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAA133990.12562810536709257No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAA132960.1246623844287531No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAA126580.11868054016991252No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAA121620.11403007817557877No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAA120440.11292371826563646No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAA119760.1122861549277036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTAA21150.034.13582635
CGGGAAG15050.032.65076429
AGTCATA23550.032.43823634
ATTCCCT10900.032.12677414
CGATACT176200.032.0903741
AATTCTG10750.031.05929434
TACGGCT10050.031.0242371
GGAAGTC15900.030.89470331
TTCATAA24850.030.88224635
GGCTTTC17800.030.658854
TCAAACA20400.030.59206835
GGACTAC4250.030.48613413
GAAGTCA109300.030.4204232
TACGGCC7100.030.3454741
CCCCGTT4050.030.2255845
ACCCCGT2200.030.2121894
ATTCTAG9250.030.04873535
CCCGATC4550.030.02587716
TTCAATG19300.029.88982234
ATTCTTA10800.029.78275535
Produced by FastQC (version 0.11.5)