FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n02_CCPD53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n02_CCPD53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15705805
Sequences flagged as poor quality0
Sequence length41
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAA293375718.67944368340241No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAA292988018.65475854309919No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAA273134417.39066542593646No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA179270411.414276441099327No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCTA2417411.5391824869849078No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCTA2387321.5200239656611043No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCTA2272771.4470891495214668No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAT2028331.2914524279398605No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTA1980171.2607886065056837No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAT1863521.1865167051290908No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAT1824981.161978007494681No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCCA1434970.9136558107018392No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAT1374000.8748357693222347No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCCA1337860.8518251691014883No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCCA1200240.7642015165730124No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTGAA844780.5378775554643649No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCGA796530.5071564303771758No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCCA787430.5013623943503692No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGAA737190.4693742218243509No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCGA718450.4574423278526634No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCGA655630.41744437805002677No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCGA598710.381203001055979No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTGAA582860.3711111910532443No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTGAA567750.3614905444197225No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGCATTCAA555970.3539901329476585No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGCATTCAA519950.3310559375975953No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGCATTCAA434950.276935820863687No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAG353580.2251269514679445No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAG349800.22272019804142484No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTGAT349680.22264379317074165No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGAT345920.22024977388933584No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGCATTCAA317290.20202084515884414No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAC316640.20160698544264363No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAC303460.1932151838126094No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAG294310.18738931242301812No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAC281260.17908028273622398No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTGAT240440.15308989255883415No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTGAT238250.1516955036688664No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAG234350.14921234537166353No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGTA218550.13915237073171355No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTGTA195960.1247691538256078No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAC175150.11151927583463567No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTGAG160350.1020960084503787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACT503.2014214E-1035.067411
CGGTGCT358.5791544E-735.067411
CGCTGCT650.035.0674061
CGATATT3900.035.0674061
CTATACT15400.035.0674061
CGTTACT6850.035.0674061
CGCTTCT750.035.0674061
CAATCCT7300.035.0674061
CATTCCT301.2015833E-535.0674061
TGATACT5500.035.0674061
ACATACT454.432877E-935.0674061
AGAGACT200.002397627635.0674061
CGAGTCT454.432877E-935.0674061
CTAAACT650.035.0674061
GGCTACT1200.035.0674061
CGGTCCT301.2015833E-535.0674061
GCATCGT200.002397627635.0674061
AGATCCT601.8189894E-1235.0674061
CGGTACT1700.035.0674061
GCATACT750.035.0674061
Produced by FastQC (version 0.11.5)