FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n02_CCPD52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n02_CCPD52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30269947
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAA348374811.508933266384643No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAA27274009.010256939002899No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAA18412746.082845140098858No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAA18243636.026977847037526No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAA17793675.878328759544904No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAA12935064.273235100147351No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAA12913004.265947343746588No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAA7624652.5188844896226614No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATA5222711.7253779796839421No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCATA4244311.4021530992439464No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCTAA3780521.248935123672334No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCTAA3164261.0453470566037No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCATA3066071.0129089423248743No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCATA2963330.9789676869933073No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCATA2759250.9115476812694782No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCTAA2719390.8983795049261236No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTAA2626360.8676460517093075No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCTAA2179660.7200739400039253No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAGA2133410.7047947589733145No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATA2118890.6999979220313799No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATA1962060.648187458009094No Hit
GATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCA1899820.6276258098502783No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAGA1791290.5917717662340142No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAGA1774420.5861985817153892No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCTAA1550330.5121680589662083No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCTTA1534480.506931842331934No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCTAA1475550.48746368799390366No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAT1462540.48316569566507667No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCACA1406720.4647249630136452No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAGA1356080.4479954986376421No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCTTA1301180.4298586978034682No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAGA1243160.4106911716759861No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAT1202830.3973677258172933No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCTTA1164910.38484044917554694No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGACTTCAAA1152850.3808562994841055No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATA1145080.3782893970709628No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCACA1125430.37179780988714645No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTTA1110240.36677963129568747No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCTAT1051310.347311476957657No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTAA984470.32523016971255353No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAT915130.30232296079011967No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGACTTCAAA905220.2990490865411823No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAGA900240.29740389039994025No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCTAT878970.29037711892921386No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCACA846570.27967343319101284No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCTTA846350.27960075384340777No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCTAT826710.27311247026630076No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAT818910.2705356570330302No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCTAG816720.2698121671636888No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCACA809060.2672816044243487No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCTAG804650.265824713865538No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAT802760.2652003321974763No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAGA796650.26318182849808097No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTAT791000.2613152907073144No Hit
GATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAAAG776180.25641934556410023No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAC771570.2548963828711031No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCACA722220.23859308376060254No Hit
GATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAG656130.2167595470186981No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGACTTCAAA654980.2163796322471262No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCTTA647880.21403407148350806No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCTAG633890.20941232569716756No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGACTTCAAA629550.20797856038532211No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAT619180.20455272022775595No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCTTA617290.20392833855969422No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGACTTCAAA616800.2037664618309375No Hit
GATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAACCA614670.20306279360185203No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAC613910.2028117194919436No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCTAT590880.19520351324037669No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAG564190.18638618693319814No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAT539880.17835511902283804No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAGA537380.17752921734550775No Hit
GATACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAG531370.17554374971320563No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTAG524590.1733039043642858No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCACA508600.16802143723608107No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCACA506370.1672847329399024No Hit
GATACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAC457140.15102107710991367No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCTAT455190.150376873801596No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGACTTCAAA454730.15022490789296725No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCTAG439370.1451505679874497No Hit
GATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAAGC439350.14514396077403108No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCTAT438640.14490940469766928No Hit
GATACTCCTTTCAAGACCTAGCTAGCTCTGTAGACTTCAAA420040.13876469621833168No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAC409300.1352166226125206No Hit
GATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAACCC392540.12967977776769812No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTTA392000.12950138300539474No Hit
GATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCTAG362840.11986806584101387No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAG361370.11938243565474363No Hit
GATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCC357130.11798170640999141No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAG345840.11425193443516765No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAC339310.11209467925398085No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCACA334680.11056510934756508No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAT324100.10706989344910316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTC601.8189894E-1235.0639421
TATACTC33850.035.0639421
GCTAATC301.2024342E-535.0639421
GCATCTC301.2024342E-535.0639421
GGTACTC10900.035.063941
GGTACTA251.6932422E-435.063941
GGTAATC454.438334E-935.063941
GAGTCTC700.035.063941
CCTACTC950.035.063941
AATATCC200.002398826235.063941
GCTTCTC2250.035.063941
GTTCGTC454.438334E-935.063941
GAAACTA251.6932422E-435.063941
CATACTG200.002398826235.063941
CATAATC1500.035.063941
TATAGTC251.6932422E-435.063941
CGTAATC251.6932422E-435.063941
AATAGTC2750.035.063941
GTAAGTC251.6932422E-435.063941
GGTTCTT358.5861393E-735.063941
Produced by FastQC (version 0.11.5)